Handbook of Experimental Laboratory Procedures

If you would like to submit an article to the HELP page please contact the Editor: James M. Downey, Ph.D., University of South Alabama MSB, Room 3074 Mobile, AL 36688 USA, jdowney@usouthal.edu.

Biochemistry

B-D protein protocols
(western blotting, kinase assays, etc,)
Invitrogen molecular biology protocols
(RT-PCR, transfection, cell culture, etc.)
Sigma Aldrich Enzyme Explorer
(contains indices, technical information and assay procedures for over 1,700 enzymes, 900 inhibitors, 600 substrates and 100 cofactors)

Electrophysiology

Measuring Single L-Type Ca Channel Activity in Isolated Heart Cells
Ira Josephson and Antonio Guia
Molecular Diversity of K+ Channels
W.A Coetzee et al
Whole-Cell Voltage Clamp of Isolated Heart Cells
Gordon M. Wahler

Isolated Cardiomyocytes

Isolation of Rabbit Cardiomyocytes
Stephen C Armstrong and Olaf Oldenburg
Isolation of Swine Cardiomyocytes
Christy Shivell and Charles Ganote
Isolation of Ventricular Myocytes from Adult Rat
Race L. Kao
Sigma Cell Culture Handbook
Broken Link...check again
The Worthington tissue dissociation guide
Introduction Tissue dissociation/primary cell isolation and cell harvesting are principal applications for enzymes in tissue culture research and cell biology studies. Despite the widespread use of enzymes for these applications over the years, their mechanisms of action in dissociation and harvesting are not well understood. As a result, the choice of one technique over another is often arbitrary and based more on past experience than on an understanding of why the method works and what modifications could lead to even better results.

Measuring Ischemic Injury

How to produce infarction in the mouse in vivo
Yiru Guo et al
Measuring infarct size by the tetrazolium method
James M. Downey

Miscellaneous

Journal of Visualized Experiments
The Journal of Visualized Experiments is an on-line journal with many useful procedures presented as short videos.

The Isolated Heart

The Isolated Blood And Perfusion Fluid Perfused Heart
Fiona J Sutherland and David J Hearse

Quick Links

Contact Us